Journal: Cell Death & Disease
Article Title: Intelligent drugs based on notch protein remodeling: a defensive targeting strategy for tumor therapy
doi: 10.1038/s41419-024-07008-7
Figure Lengend Snippet: A Fluorescence intensity at various time points following iMSC Endostatin recognition of tumors. B The endostatin-mCherry released by iMSC Endostatin at different time points was detected by a microplate reader. C The microplate reader was utilized to detect the total amount of endostatin-mCherry accumulated every four hours following full activation of iMSC Endostatin . D The amount of released endostatin by iMSC Endostatin was quantified using ELISA detection.
Article Snippet: ELISA: MSCs or iMSC Endostatin will be co-cultured with Skov3-GFP for 48 h, and MSCs and iMSC Endostatin cells will be sorted by flow cytometry and cultured in a 24-well plate coated with fibronectin for 24 h. The content of endostatin will be detected using an ELISA detection kit (CSB-E07973h; CUSABIO).
Techniques: Fluorescence, Activation Assay, Enzyme-linked Immunosorbent Assay